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KMID : 0613820110210030375
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Journal of Life Science
2011 Volume.21 No. 3 p.375 ~ p.384
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Antioxidative and Antimicrobial Activities of Methanol Extract from Rosmarinus officinalis L. and Their Fractions
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Yu Me-Hee
Chae In-Gyeong
Jung Young-Tae
Jeong Yeon-Seop
Kim Hyuk-Il
Lee In-Seon
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Abstract
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This study was performed to evaluate the antioxidant and antimicrobial activity of methanol extract from Rosmarinus officinalisL. and its fractions. The ethyl acetate fraction of rosemary had a higher antioxidant activity in both DPPH (3.22 ㎍/㎖) and ABTS (5.05 ㎍/㎖) compared to other extracts and fractions. Based on the results of the FRAP assay, the ethyl acetate fraction of rosemary showed a value of 5.9±0.3 μM/㎍, and buthanol fraction and rosmarinic acid exhibited values of 4.8±0.2 μM/㎍ and 5.1±0.1 μM/μM, respectively. Measurements of the antimicrobial activities of the extracts, fraction against gram positive, negative bacteria revealed that the methanol extract, hexane, ethyl acetate, and chloroform fraction of rosemary caused Staphylococcus aureusand Escherichia colito form clear zones greater than 12 mm. Furthermore, the methanol extract and chloroform fraction showed high antibacterial activity, with inhibition zone exceeding 13 ㎜. The methanol extract and chloroform fraction of rosemary had broad antimicrobial spectrums and low MIC values. Therefore, methanol extracts of rosemary could serve as potential antibacterial agents to inhibit pathogen growth in food and hand sanitizers.
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KEYWORD
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Antimicrobial, antioxidative, rosemary, rosmarinic acid
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